Massively parallel pyrosequencing in HIV research
نویسندگان
چکیده
منابع مشابه
Massively parallel pyrosequencing in HIV research.
Several methods for massively parallel pyrosequencing have recently been commercialized. As an example, consider the use of the 454 Life Sciences pyrosequencing method for metagenomic analysis of woolly mammoth DNA [1,2]. DNA from a mammoth carcass was purified and fragmented, and DNA linkers were ligated to the free ends. DNAs were then denatured and strands annealed to beads conjugated with o...
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Integration of retroviral DNA into host cell DNA is a defining feature of retroviral replication. HIV integration is known to be favored in active transcription units, which promotes efficient transcription of the viral genes, but the molecular mechanisms responsible for targeting are not fully clarified. Here we used pyrosequencing to map 40,569 unique sites of HIV integration. Computational p...
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ژورنال
عنوان ژورنال: AIDS
سال: 2008
ISSN: 0269-9370
DOI: 10.1097/qad.0b013e3282fc972e